Team Members: Nain, V. (Principal Investigator) and Tomar, P. (Co- PI) Research Project Title: High throughput design, synthesis and validation of TALENs for targeted Genome Engineering, Department of Biotechnology, Ministry of Science and Technology Government of India New Delhi, India.
Name of Funding Agency: Department of Biotechnology, Ministry of Science and Technology Government of India New Delhi, India. Grant Received: Rs 8628100/-
Research Project Description: With the availability of meganuclease (ZFN, TALEN & CRISPR), tailored changes can be introduced into a eukaryotic genome at any desired gene. However, IPRs held by foreign organizations, high cost of synthesis and off target activity remain major constraints in adoption of this technology for commercial purpose. The proposed research project aims to develop material (synthetic gene sequences) and methods for robust high throughput custom designed TALEN synthesis. Further, methods will be developed for analysis of genome wide activity and specificity of newly developed TALENs, using next generation sequencing.